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DOI : Background: Human Milk HM is a biological fluid representing the first nutrient for newborns. It directly impacts the development of the infant's immune system. Objective: Herein, these miRNAs were evaluated in frozen and pasteurized samples of human colostrum and HM in order to elucidate the distribution and the expression profile of these biological mediators in both biological fluids. Although we detected all the four miRNAs tested, we point out the prevalence of hsamiR- a and hsa-miR indicative to act on T and granulocytes cell populations as selective targets.